Epigenetic Research Papers 4/17/23 - 4/21/23

Alattal YZ et. al. (February 2023). Linking Histone Methylation States and hsp Transcriptional Regulation in Thermo-Tolerant and Thermo-Susceptible A. mellifera L. Subspecies in Response to Heat Stress. Insects. 14(3)
The study explored how alterations in histone methylation states are associated with hsp/hsc/trx in response to heat stress in two subspecies of honeybees, Apis mellifera jemenetica and Apis mellifera carnica. The results suggest that histone post-translational methylation may be an epigenetic mechanism of gene regulation in response to heat stress.
(Products Used: Histone H3K4me2 (H3K4 Dimethyl) Polyclonal Antibody, Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody, Histone H3K27me2 (H3K27 Dimethyl) Polyclonal Antibody, Histone H3K27me3 (H3K27 Trimethyl) Polyclonal Antibody)

Liu LL et. al. (March 2023). Choroid Plexus Modulates Subventricular Zone Adult Neurogenesis and Olfaction Through Secretion of Small Extracellular Vesicles bioRxiv. (PREPRINT)
The study discovered a regulatory axis called the CP-SVZ regulatory (CSR) axis in which the choroid plexus secretes small extracellular vesicles to regulate adult neurogenesis in the subventricular zone and maintain olfaction. The findings suggest this axis's biological and physiological presence in adult brains.
(Products Used: SMPD3 Polyclonal Antibody)

Chen L et. al. (March 2023). Regulation of endothelial-to-mesenchymal transition by histone deacetylase 3 posttranslational modifications in neointimal hyperplasia Ann Transl Med. 11(5):207.
The article investigated the effects of histone deacetylase 3 (HDAC3) on endothelial-to-mesenchymal transition (EndMT) in neointimal hyperplasia via posttranslational modifications. The results showed that HDAC3 may be involved in the regulation of EndMT through specific posttranslational modifications, suggesting a potential therapeutic target for neointimal hyperplasia.
(Products Used: EpiQuik Total Histone Extraction Kit)

Faddetta T et. al. (March 2023). Bioactive Metabolite Survey of Actinobacteria Showing Plant Growth Promoting Traits to Develop Novel Biofertilizers Metabolites. 13(3)
This article describes a study in which three actinobacterial strains were characterized for their plant growth promoting (PGP) traits and bioactive metabolites. The study found that the strains produced a rich arsenal of bioactive molecules, including antibiotics and siderophores, with Streptomyces violaceoruber being the most prolific strain, and improved the germination index and growth of tomato seedlings, suggesting their potential as eco-friendly alternatives in sustainable agriculture.
(Products Used: MethylFlash Methylated DNA Quantification Kit (Fluorometric))

Popov M et. al. (February 2023). Arsenic Soil Contamination and Its Effects on 5-Methylcytosine Levels in Onions and Arsenic Distribution and Speciation Toxics 11(3)
The article investigates the effect of arsenic-contaminated soil on onion plants and its potential impact on human health. The results indicate that the arsenic levels in the onions were highest in the roots and that the arsenic species in the soil were mainly The study also showed that increasing levels of arsenic in the soil led to a decrease in the activity of the photosynthetic apparatus in the plants.
(Products Used: MethylFlash Methylated DNA Quantification Kit (Fluorometric))

Zhang L et. al. (March 2023). HBXIP blocks myosin-IIA assembly by phosphorylating and interacting with NMHC-IIA in breast cancer metastasis Acta Pharm Sin B. 13(3):1053-1070.
The oncoprotein HBXIP promotes breast cancer cell migration by blocking the assembly of non-muscle myosin-IIA through phosphorylating and interacting with its assembly-competent domain (ACD), which is enhanced by HBXIP-recruited PKCβII kinase activity. The anti-hyperlipidemic drug bezafibrate (BZF) can be used an anti-metastatic drug for breast cancer.
(Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit)

Dong H et. al. (April 2023). HY5 functions as a systemic signal by integrating BRC1-dependent hormone signaling in tomato bud outgrowth Proc Natl Acad Sci U S A. 120(16):e2301879120.
The article describes how light regulates bud outgrowth in tomato through HY5 and brassinosteroid (BR)-dependent pathways. HY5 functions as a systemic signal by directly suppressing BRC1 transcript and activating the transcript of BR biosynthesis genes within the lateral buds, promoting bud outgrowth, while BRC1 inhibits the accumulation of cytokinin and gibberellin by inhibiting CK synthesis gene LOG4 transcript and increasing CK and GA degradation gene transcript levels, leading to bud outgrowth arrest.
(Products Used: EpiQuik Plant ChIP Kit)

McFadden T et. al. (April 2023). Controlling hypothalamic DNA methylation at the Pomc promoter does not regulate weight gain during the development of obesity PLoS One. 18(4):e0284286.
This study tested the hypothesis that epigenetic-mediated repression of the satiety gene Pomc in the hypothalamus contributes to the development of obesity. However, using the CRISPR-dCas9-TET1 and dCas9-DNMT3a systems, the authors found that controlling hypothalamic Pomc DNA methylation did not regulate weight gain during the development of obesity in male rats.
(Products Used: EpiNext CUT&RUN Fast Kit)

Lucky AB et. al. (April 2023). Characterization of the dual role of Plasmodium falciparum DNA methyltransferase in regulating transcription and translation Nucleic Acids Res.
The study investigated the function of PfDNMT2 and 5mC in the human malaria pathogen, Plasmodium falciparum, and found that PfDNMT2 plays a dual role in regulating both transcription and translation, with disruption leading to an increased proliferation phenotype and drastic alterations in gene expression. Low levels of genomic 5mC were also identified during asexual development.
(Products Used: EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Fluorometric))

An TJ et. al. (March 2023). Tiotropium Bromide Improves Neutrophilic Asthma by Recovering Histone Deacetylase 2 Activity J Korean Med Sci. 38(12):e91.
The article presents a study that suggests tiotropium bromide (TIO) improves neutrophilic asthma by recovering histone deacetylase 2 (HDAC2) activity, which is key for controlling inflammatory cytokines, and is usually downregulated in neutrophilic asthma. The study shows that TIO modulates inflammation by recovering HDAC2 activity from the acetylcholine-stimulated inflammation cascade in neutrophilic asthma, which may be associated with the PLCγ-1-IP3-IP3R mediated intracellular calcium ion pathway.
(Products Used: EpiQuik HDAC Activity/Inhibition Assay Kit (Colorimetric))

Deng K et. al. (March 2023). Targeted Demethylation of the TGFβ1 mRNA Promotes Myoblast Proliferation via Activating the SMAD2 Signaling Pathway Cells. 12(7)
The article explores the role of N6-methyladenosine (m6A) methylation in regulating the expression of transforming growth factor beta 1 (TGFβ1) during myotube differentiation, and how targeted demethylation of m6A on TGFβ1 mRNA using dCas13b-FTO can promote myoblast proliferation via activating the SMAD2 signaling pathway. The study suggests the critical role of m6A-mediated post-transcriptional regulation in myogenesis.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))

Ping Y et. al. (April 2023). Comprehensive analyses of molecular features, prognostic values, and regulatory functionalities of m6A-modified long non-coding RNAs in lung adenocarcinoma Clin Epigenetics. 15(1):60.
The article investigates the profile of m6A-modified long non-coding RNAs (lncRNAs) in lung adenocarcinoma (LUAD), finding that the dysregulation of m6A levels and regulators affects essential biological processes. The study reveals a negative correlation between expression levels and m6A modification levels in differentially m6A-modified lncRNAs and suggests a potential m6A-induced pathogenicity in LUAD.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric))

Lucky AB et. al. (April 2023). Characterization of the dual role of Plasmodium falciparum DNA methyltransferase in regulating transcription and translation Nucleic Acids Res.
The study characterized the function of PfDNMT2, the putative DNA methyltransferase in the human malaria pathogen Plasmodium falciparum. The results showed that PfDNMT2 has a dual role in regulating transcription and translation, and its disruption led to an increased proliferation phenotype and a drastic alteration in gene expression.
(Products Used: EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina))

Yoda M et. al. (April 2023). Nicotinamide-N-methyltransferase regulates lipid metabolism via SAM and 1-methylnicotinamide in the AML12 hepatocyte cell line J Biochem.
The article explores the role of Nicotinamide-N-methyltransferase (NNMT) in regulating lipid metabolism in the AML12 hepatocyte cell line. The study shows that NNMT is a significant consumer of S-adenosyl-methionine (SAM) and is critical for 1-methylnicotinamide (MNAM) production in this cell line, and that NNMT contributes to lipid metabolism by maintaining proper SAM and MNAM homeostasis.
(Products Used: EpiQuik Global Histone H3K9 Methylation Assay Kit, EpiQuik Global Histone H3K27 Methylation Assay Kit)

Park ZM et. al. (January 2023). Kar4, the Yeast Homolog of METTL14, is Required for mRNA m 6 A Methylation and Meiosis bioRxiv. (PREPRINT)
The article describes the role of KAR4, the yeast homolog of the mammalian mRNA N6A-methyltransferase complex component METTL14, in yeast mating and meiosis. The study finds that KAR4 is required for mRNA methylation during meiosis and regulates meiosis at multiple steps, including pre-meiotic DNA synthesis and sporulation.
(Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric))