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CHD1 Polyclonal Antibody

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Immunohistochemistry of paraffin-embedded mouse brain using CHD1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using CHD1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using CHD1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts from normal (control) and CHD1 knockout (KO) 293T cells, using CHD1 Polyclonal Antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using CHD1 Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 90s.
Application: IHC, WB
Areas of Research: Chromatin Remodeling Antibodies
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Knockout Validated: KO
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
Trial Size Available: A72906-020
100% Guarantee: 6 months
Catalog No.SizePriceQty
A72906-05050 µl $319.00 
A72906-100100 µl $489.00 
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Product Overview

Background
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. CHD genes alter gene expression possibly by modification of chromatin structure thus altering access of the transcriptional apparatus to its chromosomal DNA template.

Description
CHD1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
O14646

Purification
Affinity Purification

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1600 to the C-terminus of human CHD1 (NP_001261.2).

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Alternative Names
CHD1; chromodomain-helicase-DNA-binding protein 1

Application
WB, IHC; Recommended dilution: WB 1:500 - 1:2000, IHC 1:100 - 1:200

User Guide & MSDS

[User Guide]*
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