CD19 Recombinant Monoclonal Antibody [4C7]

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IHC image of CD19 rMAb diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0. 05% DAB.

Immunoprecipitating CD19 in Raji whole cell lysate
Lane 1: Rabbit control IgG instead of CD19 rMAb in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CD19 rMAb(2µg)+ Raji whole cell lysate(500µg)
Lane 3: Raji whole cell lysate (10µg)

Western Blot
Positive WB detected in: Daudi whole cell lysate, Raji whole cell lysate
All lanes: CD19 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 62 kDa
Observed band size: 95 kDa

Application:	ELISA, IHC, IP, WB
Areas of Research:	Cell Surface Receptor & Kinase
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74397-050	50µl	$299.00
A74397-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Description
CD19 Recombinant Monoclonal Antibody [4C7]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P15391

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human CD19

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
B-lymphocyte antigen CD19 (B-lymphocyte surface antigen B4) (Differentiation antigen CD19) (T-cell surface antigen Leu-12) (CD antigen CD19), CD19

Application
ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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CD19 Recombinant Monoclonal Antibody [4C7]