Epigentek Home
Cart (0)
Datasheet
Track
Account
     »    »    »    »    »   
    Quote Lookup

CD147 Recombinant Monoclonal Antibody [11F3]

Write a Review
▲
▼
The Binding Activity of CD147 with Anti-CD147 recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD147 at 2 μg/ml can bind Anti-CD147 recombinant Antibody, the EC50 is 21. 95-33. 12 ng/ml.
Overlay Peak curve showing Hela cells stained with CD147 rMAb (red line) with 1 μg/well (10 μg/mL, 100 μL/well). Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Overlay Peak curve showing Jurkat cells stained with CD147 rMAb (red line) with 1 μg/well (10 μg/mL, 100 μL/well). Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
Immunofluorescence staining of Hela cells with CD147 rMAb at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
IHC image of CD147 rMAb diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0. 05% DAB.
IHC image of CD147 rMAb diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0. 05% DAB.
IHC image of CD147 rMAb diluted at 1:200 and staining in paraffin-embedded human placenta tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0. 05% DAB.
IHC image of CD147 rMAb diluted at 1:200 and staining in paraffin-embedded human stomach tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse polymer IgG labeled by HRP and visualized using 0. 05% DAB.
Western Blot
Positive WB detected in: HepG2 whole cell lysate, ntera2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate
All lanes: CD147 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 42, 29, 23, 19 KDa
Observed band size: 35, 50-60 KDa
Application: ELISA, FC, IF, IHC, WB
Areas of Research: Cell Surface Receptor & Kinase
Clonality: Monoclonal
Conjugate: Unconjugated
Host: HEK293F Cell
Isotype: mIgG2a
Purification: Affinity Chromatography
Reactivity: Human
Recombinant: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A73941-05050µl $299.00 
A73941-100100µl $399.00 
Buy one get one free conjugated antibodiesFree Shipping on Antibodies
Order now & get it by Tuesday, October 22nd  
Product Overview

Background
The protein encoded by this gene, basigin, is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. Basigin is also a member of the immunoglobulin superfamily, ubiquitously expressed in various tissues. Multiple transcript variants encoding different isoforms have been found for this gene.

Description
CD147 Recombinant Monoclonal Antibody [11F3]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human

Isotype
mIgG2a

Uniprot ID
P35613

Purification
Affinity Chromatography

Immunogen
Recombinant Human CD147 protein

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
5A11 antigen antibody; 5F7 antibody; BASI_HUMAN antibody; Basigin (Ok blood group) antibody; Basigin antibody; Blood brain barrier HT7 antigen antibody; Bsg antibody; CD 147 antibody; CD147 antibody; CD147 antigen antibody; Collagenase stimulatory factor antibody; EMMPRIN antibody; Extracellular matrix metalloproteinase inducer antibody; Leukocyte activation antigen M6 antibody; M 6 antibody; M6 antibody; M6 leukocyte activation antigen antibody; Neurothelin antibody; OK antibody; OK blood group antibody; OK blood group antigen antibody; TCSF antibody; Tumor cell derived collagenase stimulatory factor antibody; Tumor cell-derived collagenase stimulatory factor antibody

Application
ELISA, WB, IHC, IF, FC; Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

Product FAQ  

No FAQs found at this time
Methylation Revelation
Company
About Epigentek
Press Releases
Events Calendar
Customer Feedback Survey
Corporate Sustainability
Career Opportunities
What Is Epigenetics?
Contact Us
 Ordering
Ordering Info & Return Policy
Distributors & Resellers

Support
Technical Support
Download Datasheets
Resource Bulletins
 Promotions & Offers
Current Promotions
New Lab Startup Program
Risk-Free Testing: Pioneer Program
Rewards Program
Product Reviews
Application Guarantee
Sign Up For Exclusive Offers
Request a Quote
 Stay Informed
Terms & Conditions | Privacy Policy | Site Map
Copyright © EpigenTek Group Inc. All rights reserved.
Terms & Conditions | Privacy Policy Copyright © 2020 EpiGentek