BackgroundComponent of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
DescriptionBMI1 Recombinant Monoclonal Antibody [1F2]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP35226
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Bmi1
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesPolycomb complex protein BMI-1 (Polycomb group RING finger protein 4) (RING finger protein 51), BMI1, PCGF4 RNF51
ApplicationELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200