BCL2 Polyclonal Antibody

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Western blot analysis of extracts of HL-60 cells, using BCL2 Polyclonal Antibody at 1:500 dilution.
Secondary Antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 90s.

Western blot analysis of extracts of various cells, using BCL2 Polyclonal Antibody at 1:1000 dilution.
Secondary Antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 3min.

Immunohistochemistry of paraffin-embedded human lung cancer using BCL2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using BCL2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using BCL2 Polyclonal Antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using BCL2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 cells using BCL2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using BCL2 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Application:	IF, IHC, IP, WB
Areas of Research:	Apoptosis & Cell Death
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Affinity Purified
Reactivity:	Human, Mouse, Rat
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A72934-050	50 µl	$292.00
A72934-100	100 µl	$408.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Description
BCL2 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
P10415

Purification
Affinity Purification

Immunogen
A synthetic peptide of human BCL2

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Alternative Names
BCL2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2

Application
WB, IHC, IF, IP; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:100, IF/ICC 1:50 - 1:100, IP 1:50 - 1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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BCL2 Polyclonal Antibody