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APP Polyclonal Antibody

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Western blot
All lanes: APP Polyclonal Antibody at 2.4ug/ml
Lane 1: Mouse brain tissue
Lane 2: Mouse kidney tissue
Lane 3: Mouse lung tissue
Lane 4: 293 whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: U251 whole cell lysate
Lane 7: SH-SY5Y whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 87, 35, 77, 79, 81, 83, 85, 86, 73, 84 kDa
Observed band size: 33, 50, 72, 80 kDa
Immunohistochemistry of paraffin-embedded human kidney tissue at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100
Immunofluorescent analysis of Hela cells at a dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of HepG2 cells at a dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Application: ELISA, IF, IHC, WB
Areas of Research: Signal Transduction
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A56146-02020 µg $99.00 
A56146-05050 µg $256.00 
A56146-100100 µg $359.00 
Availability: Usually Ships Within 1 Week 
Product Overview

Background
Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity By similarity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu2+-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu2+ ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Ref. 38 Ref. 65 Ref. 67 Ref. 90 Ref. 91 Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu2+ and Fe3+ to Cu+ and Fe2+, respectively. Beta-amyloid 42 is a more effective reductant than beta-amyloid 40. Beta-amyloid peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. Beta-APP42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with Also bind GPC1 in lipid rafts.

Description
APP Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
P05067

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Amyloid beta A4 protein (18-270AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, A4, AD1 , APP

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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