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AP2M1 Polyclonal Antibody

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Western blot
All lanes: AP2M1 Polyclonal Antibody at 2ug/ml
Lane 1: mouse brain tissue
Lane 2: Rat brain tissue
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 50kDa
Observed band size: 50kDa
Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100
Immunohistochemistry of paraffin-embedded human cervical cancer at dilution of 1:100
Application: ELISA, IHC
Areas of Research: Cell Trafficking
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human, Mouse, Rat
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A54500-02020 µg $99.00 
A54500-05050 µg $256.00 
A54500-100100 µg $359.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
omponent of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 By similarity. Plays a role in endocytosis of frizzled family members upon Wnt signaling

Description
AP2M1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
Q96CW1

Purification
>95%, Protein G purified

Immunogen
Recombinant Human AP-2 complex subunit mu protein (1-435AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
AP-2 mu chain Adapter-related protein complex 2 subunit mu Adaptin-mu2 Adaptor protein complex AP-2 subunit mu Clathrin assembly protein complex 2 mu medium chain Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 HA2 50 kDa subunit Plasma membrane adaptor AP-2 50 kDa protein AP2M1 CLAPM1, KIAA0109

Application
ELISA, IHC; Recommended dilution: IHC:1:20-1:200

User Guide & MSDS

[User Guide]*
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