BackgroundThe Akt kinase family includes Akt1, Akt2, and Akt3, which share similarities with protein kinase A and C families. They are encoded by the c-Akt proto-oncogene and possess Pleckstrin homology domains. Akt1 and Akt2 activation through PDGF stimulation relies on PDGFR-beta tyrosine residues 740 and 751 interacting with the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Akt1 can be activated by Insulin or Insulin-like growth factor-1 (IGF-1), leading to Thr 308 and Ser 473 phosphorylation, both essential for high Akt1 activity. Wortmannin, a PI kinase inhibitor, blocks Akt1 and Akt2 activation, indicating their role downstream of PI kinases.
DescriptionAKT1/2/3 Recombinant Monoclonal Antibody [16E10]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP31749/P31751/Q9Y243
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from Human AKT1/2/3 Antibody
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesRAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha), AKT1, PKB RAC, RAC-beta serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-2) (Protein kinase B beta) (PKB beta) (RAC-PK-beta), AKT2, RAC-gamma serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase Akt-3) (Protein kinase B gamma) (PKB gamma) (RAC-PK-gamma) (STK-2), AKT3, PKBG
ApplicationELISA, WB, IHC, FC; Recommended dilution: WB:1:500-1:2000, IHC:1:50-1:200, FC:1:50-1:200