Background This gene encodes one of the three human AKT serine-threonine protein kinases, known as protein kinase B alpha, beta, and gamma. These AKT proteins share a common structure with a pleckstrin homology domain, a serine/threonine-specific kinase domain, and a regulatory domain. They are activated by phosphoinositide 3-kinase (PI3K) and play vital roles in signaling pathways involving ligands like receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. This involvement leads to regulation of cell functions such as proliferation, survival, metabolism, and angiogenesis in normal and cancerous cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3), activating after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Full activation of AKT1 encoded by this gene requires phosphorylation of both threonine residue 308 and serine residue 473. The PI3K/AKT pathway is crucial for tumor cell survival, suppressing apoptosis and controlling the mammalian target of rapamycin (mTOR) pathway. Mutations in this gene are linked to various cancers, Proteus syndrome, Cowden syndrome 6, and excessive tissue growth. Multiple alternatively spliced transcript variants exist.
DescriptionAKT1 Recombinant Monoclonal Antibody [4B6]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman, Mouse, Rat
IsotypeRabbit IgG
Uniprot IDP31749
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human AKT1
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesRAC-alpha serine/threonine-protein kinase (EC 2.7.11.1) (Protein kinase B) (PKB) (Protein kinase B alpha) (PKB alpha) (Proto-oncogene c-Akt) (RAC-PK-alpha), AKT1, PKB RAC
ApplicationELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200