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ADAM15 Polyclonal Antibody

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Immunofluorescence analysis of HeLa cells using ADAM15 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ADAM15 Polyclonal Antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 90s.
Application: IF, WB
Areas of Research: Cellular Structure Antibodies
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
Trial Size Available: A71086-020
100% Guarantee: 6 months
Catalog No.SizePriceQty
A71086-05050 µl $256.00 
A71086-100100 µl $359.00 
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Product Overview

Background
The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Description
ADAM15 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Uniprot ID
Q13444

Purification
Affinity Purification

Immunogen
Recombinant protein of human ADAM15

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Alternative Names
ADAM15; MDC15; ADAM metallopeptidase domain 15

Application
WB, IF; Recommended dilution: WB 1:500 - 1:2000, IF 1:50 - 1:200

User Guide & MSDS

[User Guide]*
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