BackgroundTranscription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs. Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.
DescriptionZNF281 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300. 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDQ9Y2X9
PurificationAffinity purified
ImmunogenRecombinant Human Zinc finger protein 281 protein (1-300AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesZinc finger protein 281, GC-box-binding zinc finger protein 1, Transcription factor ZBP-99, Zinc finger DNA-binding protein 99, ZNF281, GZP1, ZBP99
ApplicationELISA, IHC; Recommended dilution: IHC:1:20-1:200