BackgroundMediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.
DescriptionWDYHV1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.
SpecificityHuman, Mouse
IsotypeIgG
Uniprot IDQ96HA8
Purification>95%, Protein G purified
ImmunogenRecombinant Human Protein N-terminal glutamine amidohydrolase protein (1-205AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesProtein N-terminal glutamine amidohydrolase (EC:3.5.1.-), WDYHV1, C8orf32, NTAQ1, Protein NH2-terminal glutamine deamidase, N-terminal Gln amidase, Nt(Q)-amidase, WDYHV motif-containing protein 1
ApplicationELISA, WB; Recommended dilution: WB:1:500-2000