BackgroundSIRT2 (sirtuin homologue 2) is a member of the NAD-dependent histone deacetylase family of proteins (sirtuns) which are involved in a variety of cellular functions. These functions include gene silencing, histone deacetylation, chromosomal stability, and aging. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT2 is a human homolog of the yeast SIR2 and it acts as a transcriptional silencing mediator at mating-type loci, telomeres and ribosomal gene clusters. This gene's expression rapidly increases during mitosis and is multiply phosphorylated at the G2/M transition of the cell cycle. SIRT2 is part of a phosphorylation cascade where it is phosphorylated late in G2, during M, and into the period of cytokinesis. SIRT2 appears to play an opposing role to SIRT1 in neurodegenerative diseases.
DescriptionSIRT2 Polyclonal Antibody . Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
SpecificityHuman, Mouse, Rat
IsotypeIgG
Uniprot IDQ8IXJ6
PurificationAffinity Purification
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 288-389 of human SIRT2 (NP_036369.2).
StorageShipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles
Alternative NamesSIR2, SIR2L, SIR2L2
ApplicationWB, IHC, IF; Recommended dilution: WB: 1:500-1:2000, IHC: 1:50-1:200, IF:1:10-1:100