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SIRT1 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using SIRT1 Polyclonal Antibody.
Immunohistochemistry of paraffin-embedded mouse lung using SIRT1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using SIRT1 Polyclonal Antibody.
Immunofluorescence analysis of A549 cell using SIRT1 Polyclonal Antibody. Blue: DAPI for nuclear staining.
Application: IHC
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-4700-05050 µl $189.00 
A-4700-100100 µl $299.00 
Availability: Usually Ships in 3-4 Days 
Product Overview

Background
The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging. SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53, p300, Ku70, forkhead (FoxO) transcription factors, PPARgamma, and the PPARgamma coactivator-1alpha (PGC-1alpha) protein. Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival. Deacetylation of PPARgamma and PGC-1alpha regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting. SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined.

Description
SIRT1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
Q96EB6

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human SIRT1

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
SIRT1, SIR2L1

Application
IHC

User Guide & MSDS

[User Guide]*
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Product Citations

Heo J et. al. (February 2017). Sirt1 Regulates DNA Methylation and Differentiation Potential of Embryonic Stem Cells by Antagonizing Dnmt3l. Cell Rep. 18(8):1930-1945.

Introduction to Epigenetics
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