S1PR1 Recombinant Monoclonal Antibody [4F2]

Background
This is a G-protein coupled receptor for sphingosine 1-phosphate (S1P), a bioactive lysosphingolipid. It's coupled to the G(i) subclass of G proteins. Signaling activates RAC1, SRC, PTK2/FAK1, and MAP kinases. The receptor is crucial for cell migration, especially in reorganizing the actin cytoskeleton and forming lamellipodia in response to stimuli that boost sphingosine kinase SPHK1 activity. It's essential for normal chemotaxis toward S1P, embryonic heart development, cardiac morphogenesis, sprouting angiogenesis regulation, and vascular maturation. It also aids in preventing excessive blood vessel sprouting. The receptor is necessary for mature T-cell egress from the thymus into the bloodstream and peripheral lymphoid organs. It's involved in osteoclast precursor cell migration, bone mineralization, and bone homeostasis. Additionally, it plays a role in pulmonary endothelial cell responses to oxidized lipids and safeguards against ventilator-induced lung injury.

Description
S1PR1 Recombinant Monoclonal Antibody [4F2]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P21453

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human EDG1

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Sphingosine 1-phosphate receptor 1 (S1P receptor 1) (S1P1) (Endothelial differentiation G-protein coupled receptor 1) (Sphingosine 1-phosphate receptor Edg-1) (S1P receptor Edg-1) (CD antigen CD363), S1PR1, CHEDG1 EDG1

Application
ELISA, WB; Recommended dilution: WB:1:500-1:5000