BackgroundThis gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy-terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
DescriptionRNA Polymerase II Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
SpecificityHuman, Mouse, Rat
IsotypeIgG
Uniprot IDP24928
PurificationAffinity purification
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1135-1311 of human POLR2A (NP_000928.1).
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesPOLR2A; POLR2; POLRA; RPB1; RPBh1; RPO2; RPOL2; RpIILS; hRPB220; hsRPB1; Pol II, RNA Polymerase II
ApplicationWB, IF, ICC, IP, ChIP; Recommended dilution: WB 1:500 - 1:2000, IF/ICC 1:50 - 1:200, IP 1:500 - 1:1000
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