BackgroundS1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B. pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD+ into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors.
DescriptionptxA Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation0.03% Proclin 300. 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityBordetella pertussis
IsotypeIgG
Uniprot IDP04977
PurificationProtein G purified
ImmunogenRecombinant Bordetella pertussis Pertussis toxin subunit 1 protein (35-269AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesIslet-activating protein S1 NAD-dependent ADP-ribosyltransferase ptxA BP3783
ApplicationELISA, WB; Recommended dilution: WB:1:1000-1:5000