BackgroundInhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
DescriptionPhospho-NFKBIA (S32) Recombinant Monoclonal Antibody [2D6]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDP25963
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human Phospho-NFKBIA (S32)
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesNF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
ApplicationELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200