PCYT1A Recombinant Monoclonal Antibody [4F2]

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Immunofluorescence staining of Hela Cells with PCYT1A rMAb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0. 2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: PCYT1A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa

Application:	ELISA, IF, WB
Areas of Research:	Lipid Metabolism
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74506-050	50µl	$299.00
A74506-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Controls phosphatidylcholine synthesis.

Description
PCYT1A Recombinant Monoclonal Antibody [4F2]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P49585

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human PCYT1A

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Choline-phosphate cytidylyltransferase A (EC 2.7.7.15) (CCT-alpha) (CTP:phosphocholine cytidylyltransferase A) (CCT A) (CT A) (Phosphorylcholine transferase A), PCYT1A, CTPCT PCYT1

Application
ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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PCYT1A Recombinant Monoclonal Antibody [4F2]