pan-Ubiquitin Remnant Motif (K-e-GG) Polyclonal Antibody

Background
Ubiquitination plays a key role in protein degradation and signal transduction. Characterization of ubiquitination sites is important for understanding the role of this modification in cellular processes and disease. Ubiquitination sites are usually identified by detection of Lys-e-Gly-Gly (K-e-GG)-remnant peptides, which are generated by tryptic digestion of proteomes. The di-glycine remnant left at sites of ubiquitination after trypsin digestion through cleavage of the C-terminal RGG sequence on ubiquitin (K-e-GG). This Ubiquitin K-e-GG remnant motif antibody will recognize the di-glycine remnant independent of flanking amino acid sequence.

Description
pan-Ubiquitin Remnant Motif (K-e-GG) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Human, Mouse, Rat

Isotype
IgG

Purification
Affinity Purified

Immunogen
Synthetic peptide. Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
ubiquitin remnant motif; K-e-GG; K-epsilon-GG; diGly remnant; GG remnant; ubiquitinated lysine

Application
WB, ELISA; Recommended dilution: WB, 1:500 - 1:1000; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.