p53 Polyclonal Antibody

Background
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.

Description
p53 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
P02340

Purification
Affinity Purified

Immunogen
Synthetic peptide. Please contact EpigenTek for more information.

Storage
Store at -20°C. Avoid freeze / thaw cycles.

Alternative Names
bbl; bfy; bhy; p44; p53; Tp53

Application
WB, IHC-P, IF/ICC, IP, ELISA; Recommended dilution: WB, 1:500 - 1:1000; IHC-P, 1:50 - 1:200; IF/ICC, 1:50 - 1:200; IP, 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.