NPR2 Recombinant Monoclonal Antibody [19C7]

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Overlay Peak curve showing Hela cells stained with NPR2 rMAb (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0. 2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C. Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunofluorescence staining of Hela with NPR2 rMAb at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 501-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: HEPG2 whole cell lysate, MCF7 whole cell lysate
All lanes: ANPRB antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDa

Application:	ELISA, FC, IF, WB
Areas of Research:	Cell Surface Receptor & Kinase
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A73981-050	50µl	$299.00
A73981-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Receptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.

Description
NPR2 Recombinant Monoclonal Antibody [19C7]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P20594

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from Human NPR2

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Atrial natriuretic peptide receptor 2 (EC 4.6.1.2) (Atrial natriuretic peptide receptor type B) (ANP-B) (ANPR-B) (NPR-B) (Guanylate cyclase B) (GC-B), NPR2, ANPRB

Application
ELISA, WB, IF, FC; Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200, FC:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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NPR2 Recombinant Monoclonal Antibody [19C7]