BackgroundDNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
DescriptionligA Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
Formulation0.03% Proclin 300. 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityEscherichia coli
IsotypeIgG
Uniprot IDP15042
PurificationProtein G purified
ImmunogenRecombinant Escherichia coli DNA ligase protein (1-671AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesPolydeoxyribonucleotide synthase [NAD(+)], ligA, dnaL, lig, lop, pdeC, b2411, JW2403
ApplicationELISA, WB; Recommended dilution: WB:1:1000-1:5000