BackgroundReleases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso-Dap-D-Ala. To a lesser extent, can also cleave D-Ala from murein derivatives containing the tetrapeptide, i. e. MurNAc-tetrapeptide, UDP-MurNAc-tetrapeptide, GlcNAc-MurNAc-tetrapeptide, and GlcNAc-anhMurNAc-tetrapeptide. Does not act on murein sacculi or cross-linked muropeptides. The tripeptides produced by the LcdA reaction can then be reused as peptidoglycan building blocks; LcdA is thereby involved in murein recycling. Is also essential for viability during stationary phase.
DescriptionldcA Polyclonal Antibody, Biotin Conjugated. Biotin. Raised in: Rabbit.
Formulation0.03% Proclin 300. 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityEscherichia coli
IsotypeIgG
Uniprot IDP76008
PurificationProtein G purified
ImmunogenRecombinant Escherichia coli Murein tetrapeptide carboxypeptidase protein (1-304AA)
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesLD-carboxypeptidase A, Muramoyltetrapeptide carboxypeptidase, ldcA, ycgQ, b1192, JW1181
ApplicationELISA, WB; Recommended dilution: ELISA: 1:500-1:1000, WB:1:200-1:2000