BackgroundFunctions as an E3 ubiquitin-protein ligase, transferring ubiquitin from E2 enzymes to substrates. Catalyzes 'Lys-29,' 'Lys-48,' and 'Lys-63' linked ubiquitin conjugation. Key in controlling inflammatory signaling, part of a complex (with TNFAIP3, TAX1BP1, and RNF11) that limits inflammatory pathways. Ubiquitinates RIPK1, RIPK2, NFE2, JUNB, SNX9, CXCR4, HGS/HRS, MAVS, MAP3K7, TXNIP, p15 BID, DTX1, and BRAT1. Implicated in immune response regulation, hematopoietic lineage development, TH2 cytokine development, apoptosis, and reactive oxygen species control. Modulates NOTCH1 degradation and influences MAVS-dependent antiviral responses.
DescriptionITCH Recombinant Monoclonal Antibody [9C3]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDQ96J02
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human ITCH
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesE3 ubiquitin-protein ligase Itchy homolog (Itch) (EC 2.3.2.26) (Atrophin-1-interacting protein 4) (AIP4) (HECT-type E3 ubiquitin transferase Itchy homolog) (NFE2-associated polypeptide 1) (NAPP1), ITCH
ApplicationELISA, WB; Recommended dilution: WB:1:500-1:5000