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Histone H3.1K14ac (Acetyl H3.1K14) Monoclonal Antibody [RMC418C]

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Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: Histone H3.1K14ac (Acetyl H3.1K14) Monoclonal Antibody [RMC418C] at 0.75 ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 KDa
Observed band size: 15 KDa
Immunocytochemistry analysis of Histone H3.1K14ac (Acetyl H3.1K14) Monoclonal Antibody [RMC418C] diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30min) with Histone H3.1K14ac (Acetyl H3.1K14) Monoclonal Antibody [RMC418C] at 1:46, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, ICC, IF, WB
Areas of Research: Histone Acetylation & Deacetylation
Clonality: Monoclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68403-05050 µl $249.00 
A68403-100100 µl $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
Histone H3.1K14ac (Acetyl H3.1K14) Monoclonal Antibody, clone RMC418C. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human, Rat

Isotype
IgG

Uniprot ID
P68431

Purification
Affinity Purified

Immunogen
A synthesized peptide

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA, AND, HIST1H3B, H3FL, AND, HIST1H3C, H3FC, AND, HIST1H3D, H3FB, AND, HIST1H3E, H3FD, AND, HIST1H3F, H3FI, AND, HIST1H3G, H3FH, AND, HIST1H3H, H3FK, AND, HIST1H3I, H3FF, HIST1H3J, H3FJ, Acetyl-Histone H3.1 (K14)

Application
ELISA, WB, ICC, IF; Recommended dilution: WB: 1:500-1:2000, ICC: 1:50-1:500, IF: 1:30-1:200

VCU0006

User Guide & MSDS

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