Enzyme Activity Inhibition Analysis

Enzyme Activity / Inhibition Analysis

Epigenetic regulation involves a fine-tuned balance of enzymatic activity—histone modifications like methylation and acetylation are dynamic and reversible processes controlled by specific writer and eraser enzymes. EpigenTek provides a complete set of tools to accurately measure the activity and inhibition of key epigenetic enzymes, helping researchers decipher their roles in gene regulation, chromatin dynamics, and disease development.

Our histone extraction kits offer fast, reliable isolation of total core histones from mammalian cells or tissues, with intact PTMs—ideal for use in EpigenTek’s histone modification assays or other downstream applications such as methylation, acetylation, phosphorylation, and more.

Our nuclear extraction kit enables fast, efficient isolation of high-quality nuclear proteins from cells or tissues while preserving enzymatic activity—ideal for use in our histone modification assays and a wide range of downstream applications.

Explore Our Powerful Solutions for In Vivo Protein-DNA Interactions

Histone Methyltransferase (HMT) Assays: Histone methyltransferases catalyze the methylation of lysine, arginine, and other residues on histone proteins. These methylation marks influence chromatin structure and gene expression patterns. Aberrant HMT activity is implicated in cancer, neurological disorders, and more, making them prime drug targets.

Histone Demethylase (HDM) Assays: Histone demethylases dynamically remove methyl groups from histones, reversing previously thought irreversible epigenetic marks. Studying HDMs is essential for understanding gene reprogramming and pathological states like cancer.

Histone Acetyltransferase (HAT) Assay: Histone acetyltransferases are enzymes that transfer acetyl groups from acetyl-coenzyme A to the lysine residues of histones. These enzymes play important roles in regulating gene expression by modifying the structure of chromatin, making it more accessible for transcription.

Histone Deacetylase (HDAC) Assays: HDACs remove acetyl groups from histones, promoting chromatin condensation and gene repression. Their dysregulation is associated with cancer, neurodegeneration, and metabolic diseases. HDAC inhibitors are an important class of therapeutic drugs under active investigation.

Featured Kits

Histone Methyltransferase (HMT) Assays
Click image to see more details	EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K4) Very rapid procedure, which can be completed within 3 hours Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography Specific measurement of activity/inhibition of H3-K4 histone methyltransferases Strip microplate format makes the assay flexible: manual or high throughput analysis Extremely simple, reliable, and consistent assay conditions Catalog #: P-3002
Click image to see more details	EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K9) Very rapid procedure, which can be completed within 3 hours Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography Specific measurement of activity/inhibition of H3-K9 histone methyltransferases Strip microplate format makes the assay flexible: manual or high throughput analysis Extremely simple, reliable, and consistent assay conditions Catalog #: P-3003
Click image to see more details	EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K27) Very rapid procedure, which can be completed within 3 hours Safe and innovative colorimetric assay without radioactivity, electrophoresis, and chromatography Specific measurement of activity/inhibition of H3-K27 histone methyltransferases Strip microplate format makes the assay flexible: manual or high throughput analysis Extremely simple, reliable, and consistent assay conditions Catalog #: P-3005
Histone Demethylase (HDM) Assays
Click image to see more details	Epigenase LSD1 Demethylase Activity/Inhibition Assay Kit (Fluorometric) 3-hour procedure in 96-stripwell format for manual or high-throughput analysis Directly detects LSD1-converted demethylated products, avoiding interference from thiol-containing compounds Compatible with cell/tissue extracts or purified LSD1 for in vivo/in vitro inhibitor screening Highly sensitive—detects as low as 2 ng LSD1, with included H3-K4 standard for activity quantification Catalog #: P-3079
Click image to see more details	Epigenase JMJD2 Demethylase Activity/Inhibition Assay Kits 3-hour procedure in 96-stripwell format for manual or high-throughput use Directly detects JMJD2 activity via demethylated products, avoiding interference from thiols, detergents, and ions Compatible with cell/tissue extracts or purified JMJD2 for in vivo/in vitro inhibitor testing Ultra-sensitive—detects activity from as little as 10 ng of enzyme, with included H3-K9 standard for activity quantification Catalog #s: P-3080 / P-3081
Histone Acetyltransferase (HAT) Assay
Click image to see more details	EpiQuik HAT Activity/Inhibition Assay Kit Fast 3-hour procedures Directly measures HAT activity and inhibition Colorimetric assay with no radioactivity or extraction Flexible strip-well microplate format Catalog #: P-4003
Histone Deacetylase (HDAC) Assays
Click image to see more details	Epigenase HDAC Activity/Inhibition Direct Assay Kits Quick 3.5-hour procedure Detects as little as 0.5 ng of purified HDAC enzyme Detection of HDAC inhibition in vivo or in vitro Flexible strip-well microplate format Catalog #s: P-4034 / P-4035
Click image to see more details	Epigenase Universal SIRT Activity/Inhibition Assay Kits Quick 3.5-hour procedure Detects as little as 1 ng of purified SIRT enzyme Detection of SIRT inhibition in vivo or in vitro Flexible strip-well microplate format Catalog #s: P-4036 / P-4037