Comprehensive Solutions for In Vivo Protein‑DNA Interaction

In Vivo Protein-DNA Interaction

EpigenTek provides powerful solutions to study in vivo protein-DNA interactions, helping researchers analyze transcription factor/histone binding sites and chromatin structure with precision and efficiency.

Our chromatin isolation kits offer fast and reliable chromatin extraction from cells or tissues, ensuring the preservation of protein-DNA interactions for downstream applications such as ChIP, ChIP-seq, and CUT&RUN-based assays.

Explore Our Powerful Solutions for In Vivo Protein-DNA Interactions

Chromatin Immunoprecipitation (ChIP): A widely used technique to study protein-DNA interactions, EpigenTek’s ChIP kits offer high specificity using antibodies targeted to proteins of interest. Our optimized protocols overcome common challenges such as high sample input requirements and lengthy processing times, ensuring fast and reproducible results.

ChIP-seq: Take your research to the next level with our ChIP-seq solutions, enabling genome-wide mapping of protein-DNA interactions with high resolution and coverage. Designed for compatibility with next-generation sequencing (NGS), our kits provide a comprehensive view of protein binding sites with minimal background noise and optimized workflows.

CUT&RUN Fast and More: Experience unparalleled speed and precision with our CUT&RUN-based solutions, offering significant advantages over traditional ChIP methods.

CUT&RUN-Fast (Cleavage Under Target & Release Using Nuclease Fast): This enhanced technique employs a novel nucleic acid cleavage enzyme mix with low sequence bias. It simultaneously fragments chromatin and removes non-specific DNA sequences at both ends of the target protein/DNA complex, increasing specificity and resolution while significantly reducing processing time by eliminating overnight incubation.
cTIP (CUT&Tag In-Place): An all-in-one solution combining protein-DNA complex enrichment and NGS library generation for high resolution chromatin profiling.
CUT&LUNCH (Cleavage Under Target and Liberate Unique Nucleic Complex Homogenously): Developed exclusively by EpigenTek, this innovative kit combines the benefits of ChIP and CUT&RUN while eliminating their drawbacks. It offers highly specific protein/DNA enrichment in record time with enhanced specificity and minimal background—be done by lunch!
CUT&LUNCH-Seq Kit: The NGS-optimized version of CUT&LUNCH, designed for seamless high-resolution protein-DNA interaction analysis with compatibility for standard ChIP-seq bioinformatics pipelines.

These methods help in understanding regulatory networks, epigenetic modifications, and transcriptional control mechanisms inside living cells or tissues.

Featured Kits

Chromatin Immunoprecipitation (ChIP)
Click image to see more details	EpiQuik Chromatin Immunoprecipitation (ChIP) Kit For immunoprecipitating chromatin specifically from cell input samples via microplate format Quick 5-hour microplate-based procedure Includes DNA purification columns Compatible with diverse DNA amplification methods Consistently reliable assay conditions Catalog #: P-2002
Click image to see more details	ChromaFlash High-Sensitivity ChIP Kit For immunoprecipitation of chromatin (ChIP) from small amounts of mammalian cells or tissues Quick 5-hour microplate procedure Optimized for minimal background, increased sensitivity Requires a minimum of just 2,000 cells per ChIP reaction Compatible with ChIP-PCR, ChIP-on-chip, and ChIP-seq analyses Catalog #: P-2027
Click image to see more details	EpiQuik Plant ChIP Kit For immunoprecipitating chromatin specifically from plant input samples Fast 6-hour microplate procedure Includes DNA purification columns Compatible with diverse DNA amplification methods Consistently reliable assay conditions Catalog #: P-2014
ChIP-seq
Click image to see more details	EpiNext ChIP-Seq High-Sensitivity Kit (Illumina) For streamlined ChIP sequencing NGS library preparation from low input cells Streamlined <7-hour microplate procedure Optimized for minimal background, increased sensitivity Contains all ChIP-seq components for reliable results Minimized bias with Ultra HiFi amplification, low error rates Catalog #: P-2030
CUT&RUN Fast and More
Click image to see more details	EpiNext CUT&LUNCH Assay Kit For ultra-fast, highly specific enrichment of target protein-DNA complexes Complete your assay in less than 2 hours Only 3-5% non-specific DNA binding DNA is selectively cleaved at both ends of target protein-enriched regions Nearly half the price per reaction compared to traditional CUT&RUN assays Use existing, time-tested ChIP-seq bioinformatics pipelines for NGS data analysis Catalog #: P-2035
Click image to see more details	EpiNext CUT&LUNCH-Seq Kit For ultra-fast, highly specific enrichment of target protein-DNA complexes with streamlined library prep all-in-one Complete your assay in less than 3 hours High specificity with low background using selective DNA cleavage for precise mapping Requires low input and maximum protein protection All-in-one, cost-effective kit for a streamlined workflow NGS-ready and compatible with standard ChIP-seq tool Catalog #: P-2033
Click image to see more details	EpiNext CUT&RUN Fast Kit For preparing samples for protein-DNA interaction analysis with better signal-to-noise ratio than ChIP Extremely fast 3-hour procedure from cells to enriched DNA Higher enrichment versus traditional ChIP Input amount can be as low as 500 cells or 50 ng of chromatin High signal-to-noise (S/N) ratio Catalog #: P-2028
Click image to see more details	EpiNext cTIP (CUT&Tag In-Place)-Sequencing Kit For high resolution chromatin profiling with all-in-one protein-DNA complex enrichment and NGS library generation Fast 5-hour procedure from cells to library DNA Higher enrichmentversus traditional ChIP Input amount can be as low as 500 cells or 50 ng of chromatin High signal-to-noise (S/N) ratio Includes library construction step Catalog #: P-2032