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EHMT2 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using EHMT2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Application: IF, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3019-05050 µl $199.00 
A-3019-100100 µl $391.00 
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Product Overview

Background
EHMT2 (Euchromatic Histone Lysine Methyltransferase 2) is a histone methyltransferase that specifically mono- and dimethylates Lys-9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me is a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. This gene also mediates monomethylation of Lys-56 of histone H3 (H3K56me1) in G1 phase, promoting interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates Lys-27 of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of Lys-373 of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Description
EHMT2 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3..

Specificity
Mouse, Rat, Human

Isotype
IgG

Uniprot ID
Q96KQ7

Purification
Affinity Purified

Immunogen
Recombinant Protein of Human EHMT2

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
EHMT2; BAT8; C6orf30; G9A; GAT8; KMT1C; NG36; histone-lysine N-methyltransferase EHMT2

Application
WB, IF; Recommended dilution: WB 1:500 - 1:2000;IF 1:50 - 1:100

User Guide & MSDS

[User Guide]*
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