EHMT2 Polyclonal Antibody

Background
EHMT2 (Euchromatic Histone Lysine Methyltransferase 2) is a histone methyltransferase that specifically mono- and dimethylates Lys-9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me is a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. This gene also mediates monomethylation of Lys-56 of histone H3 (H3K56me1) in G1 phase, promoting interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates Lys-27 of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of Lys-373 of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Description
EHMT2 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human

Isotype
IgG

Uniprot ID
Q96KQ7

Purification
Affinity Purified

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5).

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze.

Alternative Names
EHMT2; BAT8; C6orf30; G9A; GAT8; KMT1C; NG36; histone-lysine N-methyltransferase EHMT2

Application
WB, IP, ELISA; Recommended dilution: WB 1:500 - 1:1000, IP 0.5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells