BackgroundThis gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
DescriptionDNA topoisomerase I (TOP1) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationBuffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
SpecificityHuman, Mouse
IsotypeIgG
Uniprot IDP11387
PurificationAffinity Purified
ImmunogenSynthetic peptide. Please contact EpigenTek for more information.
StorageStore at -20°C. Avoid freeze / thaw cycles.
Alternative NamesTOPI; DNA topoisomerase I (TOP1)
ApplicationWB, IHC-P, IP, ELISA; Recommended dilution: WB, 1:500 - 1:2000; IHC-P, 1:50 - 1:200; IP, 0.5µg-4µg antibody for 200µg-400µg extracts of whole cells; ELISA, Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
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