DGCR8 Recombinant Monoclonal Antibody [2H2]

Background
Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing. Involved in the silencing of embryonic stem cell self-renewal (By similarity).

Description
DGCR8 Recombinant Monoclonal Antibody [2H2]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
Q8WYQ5

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human DGCR8

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Microprocessor complex subunit DGCR8, DiGeorge syndrome critical region 8, DGCR8, C22orf12, DGCRK6, LP4941

Application
ELISA, WB; Recommended dilution: WB:1:500-1:5000