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CENPJ Polyclonal Antibody

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Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: CENPJ antibody at 3.2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 154, 125 KDa
Observed band size: 125 KDa
Immunofluorescence staining of HepG2 cells with CENPJ Polyclonal Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, WB
Areas of Research: Cell Cycle
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69435-02020 µg $99.00 
A69435-05050 µg $249.00 
A69435-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Plays an important role in cell division and centrosome function by participating in centriole duplication. Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles. Required for centriole elongation and for STIL-mediated centriole amplification. May be involved in the control of centriolar-microtubule growth by acting as a regulator of tubulin release.

Description
CENPJ Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
Q9HC77

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Centromere protein J protein (1082-1248AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Centromere protein J, CENP-J, Centrosomal P4.1-associated protein, LAG-3-associated protein, LYST-interacting protein 1, CENPJ, CPAP, LAP, LIP1

Application
ELISA, WB, IF; Recommended dilution: WB: 1:500-1:5000, IF: 1:50-1:200

User Guide & MSDS

[User Guide]*
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