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ATG4D Polyclonal Antibody

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Western Blot
Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, Mouse heart tissue
All lanes: ATG4D Polyclonal Antibody at 2.8ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53, 17 KDa
Observed band size: 53 KDa
Immunohistochemistry of paraffin-embedded human small intestine tissue using ATG4D Polyclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human pancreatic tissue using ATG4D Polyclonal Antibody at dilution of 1:100.
Immunofluorescent analysis of PC3 cells using ATG4D Polyclonal Antibody at a dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC, WB
Areas of Research: Apoptosis & Cell Death
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: >95%, Protein G Purified
Reactivity: Human, Mouse
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A65918-02020 µg $99.00 
A65918-05050 µg $256.00 
A65918-100100 µg $359.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Description
ATG4D Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, PH 7.4.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
Q86TL0

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Cysteine protease ATG4D protein (14-43AA)

Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, ATG4D, APG4D, AUTL4

Application
ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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