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ALOX15B Polyclonal Antibody

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Immunofluorescence analysis of MCF7 cells using ALOX15B Polyclonal Antibody.
Western blot analysis of extracts of various cell lines, using ALOX15B Polyclonal Antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Exposure time: 30s.
Application: IF, WB
Areas of Research: Lipid Metabolism Antibodies
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human, Mouse
Trial Size Available: A71771-020
100% Guarantee: 6 months
Catalog No.SizePriceQty
A71771-05050 µl $256.00 
A71771-100100 µl $359.00 
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Product Overview

Background
This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described.

Description
ALOX15B Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
O15296

Purification
Affinity Purification

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ALOX15B (NP_001132.2).

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid freeze / thaw cycles

Alternative Names
ALOX15B; 15-LOX-2; arachidonate 15-lipoxygenase B

Application
WB, IF; Recommended dilution: WB 1:500 - 1:2000, IF 1:50 - 1:200

User Guide & MSDS

[User Guide]*
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