RNF213 Polyclonal Antibody

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IHC image of RNF213 Polyclonal Antibody diluted at 1:800 and staining in paraffin-embedded human gastric cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of RNF213 Polyclonal Antibody diluted at 1:800 and staining in paraffin-embedded human colon cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with RNF213 Polyclonal Antibody at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Application:	ELISA, IF, IHC
Areas of Research:	Cell Metabolism
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Protein G Purified
Reactivity:	Human
Trial Size Available:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A68923-020	20 µl	$111.00
A68923-050	50 µl	$277.00
A68923-100	100 µl	$445.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
E3 ubiquitin-protein ligase involved in angiogenesis. Involved in the non-canonical Wnt signaling pathway in vascular development: acts by mediating ubiquitination and degradation of FLNA and NFATC2 downstream of RSPO3, leading to inhibit the non-canonical Wnt signaling pathway and promoting vessel regression. Also has ATPase activity.

Description
RNF213 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4

Specificity
Human

Isotype
IgG

Uniprot ID
Q63HN8

Purification
Protein G purified

Immunogen
Recombinant Human E3 ubiquitin-protein ligase RNF213 protein (202-350AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
E3 ubiquitin-protein ligase RNF213, ALK lymphoma oligomerization partner on chromosome 171, RNF213

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:500-1:1000, IF:1:200-1:500

Datasheet & SDS

Datasheet/User Guide
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RNF213 Polyclonal Antibody