BackgroundVariant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code and nucleosome remodeling.
DescriptionPhospho Histone H3.3 (S31) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationPBS with 50% glycerol, 0.5% BSA and 0.02% sodium azide.
SpecificityHuman, Mouse, Rat
IsotypeIgG
Uniprot IDP84243
PurificationAffinity Purified
ImmunogenSynthetic Peptide
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesH3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3.3S31; H3.3S31ph
ApplicationIHC, IF, ELISA; Recommended dilution: IHC:1:100-1:300, IF:1:200-1:1000, ELISA:1:20000