KAT8 Recombinant Monoclonal Antibody [7E11]

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Immunofluorescence staining of HepG2 cell with KAT8 rMAb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 587-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IHC image of KAT8 rMAb diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Application:	ELISA, IF, IHC
Areas of Research:	Histone Acetylation & Deacetylation
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A73867-050	50µl	$299.00
A73867-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.

Description
KAT8 Recombinant Monoclonal Antibody [7E11]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
Q9H7Z6

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human KAT8

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Histone acetyltransferase KAT8 (EC 2.3.1.48) (Lysine acetyltransferase 8) (MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1) (MYST-1) (hMOF), KAT8, MOF MYST1

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:50-1:200, IF:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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KAT8 Recombinant Monoclonal Antibody [7E11]