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HIST1H3A (K79) Acetyl Polyclonal Antibody

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Immunofluorescence staining of Hela cells with HIST1H3A (K79) Acetyl Polyclonal Antibody at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF
Areas of Research: Histone Modification
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A70363-05050 µl $249.00 
A70363-100100 µl $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
HIST1H3A (K79) Acetyl Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Concentration
Inquire

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P68431

Purification
Affinity Purified

Immunogen
Peptide sequence around site of acetyl-Lys?79? derived from human Histone H3

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, HIST1H3A, H3FA, AND, HIST1H3B, H3FL, AND, HIST1H3C, H3FC, AND, HIST1H3D, H3FB, AND, HIST1H3E, H3FD, AND, HIST1H3F, H3FI, AND, HIST1H3G, H3FH, AND, HIST1H3H, H3FK, AND, HIST1H3I, H3FF, AND, HIST1H3J, H3FJ

Application
ELISA, IF; Recommended dilution: IF: 1:50-1:200

VCU0006

User Guide & MSDS

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