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HIST1H2AG Monomethyl (R29) Polyclonal Antibody

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Immunofluorescence staining of Hela cells with HIST1H2AG Monomethyl (R29) Polyclonal Antibody, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF
Areas of Research: Histone Modification
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Human
100% Guarantee: 6 months
Catalog No.SizePriceQty
A69360-05050 µl $249.00 
A69360-100100 µl $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Description
HIST1H2AG Monomethyl (R29) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P0C0S8

Purification
Affinity purified

Immunogen
Peptide sequence around site of Mono-methyl-Arg(29) derived from Human Histone H2A

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Histone H2A type 1, H2A.1, Histone H2A/p, HIST1H2AG, H2AFP, AND, HIST1H2AI, H2AFC, AND, HIST1H2AK, H2AFD, AND, HIST1H2AL, H2AFI, AND, HIST1H2AM, H2AFN

Application
ELISA, IF; Recommended dilution: IF: 1:50-1:200

User Guide & MSDS

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