GZMB Recombinant Monoclonal Antibody [6A1]

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Immunofluorescence staining of Hela Cells with GZMB rMAb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0. 2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

IHC image of GZMB rMAb diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6. 0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0. 05% DAB.

Application:	ELISA, IF, IHC
Areas of Research:	Immunology & Inflammation
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A74349-050	50µl	$299.00
A74349-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Description
GZMB Recombinant Monoclonal Antibody [6A1]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P10144

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from human Granzyme B

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
Granzyme B (EC 3.4.21.79) (C11) (CTLA-1) (Cathepsin G-like 1) (CTSGL1) (Cytotoxic T-lymphocyte proteinase 2) (Lymphocyte protease) (Fragmentin-2) (Granzyme-2) (Human lymphocyte protein) (HLP) (SECT) (T-cell serine protease 1-3E), GZMB, CGL1 CSPB CTLA1 GRB

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:50-1:200, IF:1:20-1:200

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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GZMB Recombinant Monoclonal Antibody [6A1]