GCN5 Polyclonal Antibody

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IHC image of GCN5 Polyclonal Antibody diluted at 1:66 and staining in paraffin-embedded human pancreatic tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then, primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of GCN5 Polyclonal Antibody diluted at 1:66 and staining in paraffin-embedded human melanoma cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then, primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB

Immunofluorescence staining of Hela cell with GCN5 Polyclonal Antibody at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Application:	ELISA, IF, IHC
Areas of Research:	Histone Acetylation & Deacetylation
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Affinity Purified
Reactivity:	Human
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A-4013-050	50 µl	$222.00
A-4013-100	100 µl	$362.00

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Datasheet & SDS
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Product Overview

Background
GCN5, a putative transcriptional adapter in humans and yeast, possesses histone acetyltransferase (HAT) activity. GCN5 HATs operate in multisubunit complexes (e.g., SAGA and ADA) to acetylate lysine residues in histone tails, preferentially those of histone H3. Also, GCN5 is required for the activation of a number of genes involved in amino acid biosynthesis, respiration, and other pathways controlled by the SWI/SNF gene products.

Description
GCN5 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Specificity
Human

Isotype
IgG

Uniprot ID
Q92830

Purification
Affinity Purified

Immunogen
Synthesized peptide derived from the C-terminal region of human GCN5.

Storage
Store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
KAT2A; GCN5; GCN5L2; HGCN5; Histone acetyltransferase KAT2A; General control of amino acid synthesis protein 5-like 2; Histone acetyltransferaseGCN5; HsGCN5; Lysine acetyltransferase 2A; STAF97

Application
IF, IHC, ELISA; Recommended Dilution; IF:1:20-1:300, IHC:1:100-1:300, ELISA:1:20000

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

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GCN5 Polyclonal Antibody