Epigenetic Research Papers 7/31/23 - 8/4/23

Bazan N et. al. (July 2023). Elovanoids are neural resiliency epigenomic regulators targeting histone modifications, DNA methylation, tau phosphorylation, telomere integrity, senescence programming, and dendrite integrity Res Sq.
The article discusses the role of elovanoids (ELVs), mediators derived from very-long-chain polyunsaturated fatty acids (VLC-PUFAs), in regulating various aspects of epigenomic regulation in neurons. ELVs are shown to counteract damage caused by different insults, targeting histone modifications, DNA methylation, tau phosphorylation, telomere integrity, senescence programming, and dendrite integrity, indicating their role as neural resiliency epigenomic regulators.
Products Used: EpiQuik Total Histone Extraction Kit, EpiQuik Nuclear Extraction Kit, EpiQuik Global Pan-Methyl Histone H3K9 Quantification Kit (Colorimetric), EpiQuik Global Tri-Methyl Histone H3K27 Quantification Kit (Colorimetric), EpiQuik Global Acetyl Histone H3K9 Quantification Kit (Colorimetric), EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric), MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric), EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit, EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric), Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric)

Liu Y et. al. (July 2023). An Fgr kinase inhibitor attenuates sepsis-associated encephalopathy by ameliorating mitochondrial dysfunction, oxidative stress, and neuroinflammation via the SIRT1/PGC-1α signaling pathway J Transl Med. 21(1):486.
The article explores the potential of an Fgr kinase inhibitor in treating sepsis-associated encephalopathy (SAE) and investigates its underlying mechanisms. The Fgr inhibitor showed promise in reducing neuroinflammation, oxidative stress, and mitochondrial dysfunction in a mouse model of sepsis, and its protective effects were mediated through the SIRT1/PGC-1α signaling pathway.
Products Used: Epigenase Universal SIRT Activity/Inhibition Assay Kit (Colorimetric), EpiQuik Nuclear Extraction Kit

Scholpa NE et. al. (July 2023).  Role of DNA methylation during recovery from spinal cord injury with and without β2-adrenergic receptor agonism Exp Neurol. :114494.
The study investigates the role of DNA methylation in recovery from spinal cord injury (SCI) with and without treatment using the β2-adrenergic receptor agonist formoterol. The researchers found that DNA methylation plays a crucial role in the recovery process, and treatment with the DNA methyltransferase inhibitor decitabine (DAC) led to improved functional recovery and reduced pain response in injured mice, supporting both DNMT inhibition and formoterol as potential therapeutic approaches for SCI.
Products Used: EpiQuik Nuclear Extraction Kit, EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric)

Yang Y et. al. (July 2023). METTL3-dependent m6A modification mediates bladder remodeling after partial bladder outlet obstruction through CCN2 activation Neurourol Urodyn.
The study investigates the role of N6-methyladenosine (m6A) modification in bladder remodeling after partial bladder outlet obstruction (pBOO). The researchers identified METTL3-dependent m6A modification as a critical mechanism in pBOO-induced bladder remodeling and highlighted CCN2 as a potential pharmaceutical target for future therapies in pBOO patients.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Park J et. al. (July 2023). Poly(GR) interacts with key stress granule factors promoting its assembly into cytoplasmic inclusions Cell Rep. 42(8):112822.
The article investigates the formation of cytoplasmic inclusions containing Poly(GR) proteins, which are toxic to neurons and associated with frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). The study reveals that Poly(GR) interacts with key stress granule factors, G3BP1 and YTHDF proteins, promoting its assembly into cytoplasmic inclusions and suggests potential therapeutic strategies for c9FTD/ALS treatment.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Ensinck I et. al. (July 2023). The yeast RNA methylation complex consists of conserved yet reconfigured components with m6A-dependent and independent roles Elife. 12
The study explores the yeast RNA methylation complex (MTC) and its role in mRNA modification. The MTC consists of conserved yet reconfigured components, with some proteins playing m6A-dependent roles, others exerting m6A-independent functions, and some contributing to both, highlighting the complex and multifunctional nature of the MTC in yeast and providing insights into potential similarities in mammalian MTC functions.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Meng X et. al. (July 2023). Microcystin-LR induces lactate production disruption via altering the m6A modification in Sertoli cells Ecotoxicol Environ Saf. 263:115288.
The article investigates the impact of microcystin-LR (MC-LR) on the male reproductive system, particularly on lactate production in Sertoli cells. The study shows that MC-LR disrupts lactate production by suppressing the expression of lactate dehydrogenase A (Ldha) through N6-methyladenosine (m6A) methylation catalyzed by methyltransferase 3 (Mettl3), leading to spermatogenic cell apoptosis and decreased sperm quality, providing insights into the mechanism of MC-LR-induced male infertility.
Products Used: EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Srivastava S et. al. (July 2023). Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism Nat Commun. 14(1):4360.
The study investigates the adaptive response of cardiomyocytes to anthracycline-induced cardiotoxicity, revealing that selected mitochondrial tricarboxylic acid (TCA) cycle dehydrogenases translocate to the nucleus as a protective response. This nuclear translocation of mitochondrial dehydrogenases helps maintain their function by altering the nuclear metabolic milieu through metabolite-induced chromatin accessibility and AMP-kinase (AMPK) signaling, providing insights into potential strategies to attenuate chemotherapy-induced cardiac damage.
Products Used: EpiQuik Chromatin Accessibility Assay Kit

Zhang R et. al. (July 2023). A positive feedback loop between miR-574-3p and HIF-1α in promoting angiogenesis under hypoxia Microvasc Res. 150:104589.
The study explores a positive feedback loop between miR-574-3p and HIF-1α in promoting angiogenesis under hypoxic conditions. MiR-574-3p downregulates cullin 2 (CUL2), leading to the stabilization of HIF-1α and VEGF, which enhances angiogenesis, cell proliferation, migration, and invasion in gastric cancer cells and endothelial cells.
Products Used: EpiQuik Chromatin Immunoprecipitation (ChIP) Kit