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CYBA Polyclonal Antibody

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Immunofluorescence staining of MCF-7 cells with CYBA Polyclonal Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application: ELISA, IF, IHC
Areas of Research: Signal Transduction
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Protein G Purified
Reactivity: Human
Trial Size Available: Yes
100% Guarantee: 6 months
Catalog No.SizePriceQty
A68507-02020 µg $99.00 
A68507-05050 µg $249.00 
A68507-100100 µg $399.00 
Availability: Usually Ships Within 1-2 Weeks 
Product Overview

Background
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Description
CYBA Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.

Specificity
Human

Isotype
IgG

Uniprot ID
P13498

Purification
>95%, Protein G purified

Immunogen
Recombinant Human Cytochrome b-245 light chain protein (128-195AA)

Storage
Shipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.

Alternative Names
Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox, p22phox, CYBA

Application
ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200

User Guide & MSDS

[User Guide]*
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