BackgroundEssential non-receptor tyrosine kinase for B lymphocyte development, differentiation, and signaling. Activated by B-cell antigen receptor (BCR) engagement, BTK phosphorylates PLCG2 to initiate calcium mobilization and activate protein kinase C (PKC) family members via the BLNK adapter protein. BTK's role extends to cytokine receptor signaling, innate and adaptive immunity, and Toll-like receptor (TLR) pathways. It critically regulates TLR9 activation and TIRAP degradation, influencing NF-kappa-B-mediated transcription of genes related to immune response. BTK phosphorylates transcription factors GTF2I, ARID3A, and NFAT, which impact gene expression and ARID3A DNA-binding complex formation. BTK also has a dual role in apoptosis regulation.
DescriptionBTK Recombinant Monoclonal Antibody [3E5]. Unconjugated. Raised in: HEK293F Cell.
FormulationBuffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
SpecificityHuman
IsotypeRabbit IgG
Uniprot IDQ06187
PurificationAffinity Chromatography
ImmunogenA synthesized peptide derived from human BTK
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Alternative NamesTyrosine-protein kinase BTK (EC 2.7.10.2) (Agammaglobulinemia tyrosine kinase) (ATK) (B-cell progenitor kinase) (BPK) (Bruton tyrosine kinase), BTK, AGMX1 ATK BPK
ApplicationELISA, IHC; Recommended dilution: IHC:1:50-1:200