BackgroundNegatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
DescriptionBRAP Polyclonal Antibody, HRP Conjugated. HRP. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDQ7Z569
Purification>95%, Protein G purified
ImmunogenRecombinant Human BRCA1-associated protein (13-124AA)
StorageShipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesBRCA1-associated protein, BRAP2, Impedes mitogenic signal propagation, IMP, RING finger protein 52, RING-type E3 ubiquitin transferase BRAP2, Renal carcinoma antigen NY-REN-63, BRAP
ApplicationELISA; Recommended dilution: ELISA 1:500-1:1000