WDR5 Recombinant Monoclonal Antibody [18D11]

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Overlay Peak curve showing Hela cells stained with WDR5 rMAb (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

IHC image of WDR5 rMAb diluted at 1:50 and staining in paraffin-embedded human testis tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.79% DAB.

Application:	ELISA, FC, IHC
Areas of Research:	Histone Methylation
Clonality:	Monoclonal
Conjugate:	Unconjugated
Host:	HEK293F Cell
Isotype:	Rabbit IgG
Purification:	Affinity Chromatography
Reactivity:	Human
Recombinant:	Yes
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A73880-050	50µl	$299.00
A73880-100	100µl	$399.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Background
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified.

Description
WDR5 Recombinant Monoclonal Antibody [18D11]. Unconjugated. Raised in: HEK293F Cell.

Formulation
Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Specificity
Human

Isotype
Rabbit IgG

Uniprot ID
P61964

Purification
Affinity Chromatography

Immunogen
A synthesized peptide derived from Human WDR5

Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names
WD repeat-containing protein 5 (BMP2-induced 3-kb gene protein), WDR5, BIG3

Application
ELISA, IHC, FC; Recommended dilution: IHC:1:50-1:200, FC:1:50-1:200

Datasheet & SDS

Datasheet/User Guide
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WDR5 Recombinant Monoclonal Antibody [18D11]