BackgroundE3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1.
DescriptionTRIM71 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDQ2Q1W2
Purification>95%, Protein G purified
ImmunogenRecombinant Human E3 ubiquitin-protein ligase TRIM71 protein (125-209AA)
StorageShipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesE3 ubiquitin-protein ligase TRIM71, Protein lin-41 homolog, RING-type E3 ubiquitin transferase TRIM71, Tripartite motif-containing protein 71, TRIM71, LIN41
ApplicationELISA, IHC; Recommended dilution: IHC: 1:500-1:1000