BackgroundComponent of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules. In the complex, it mediates the interaction with microtubules.
DescriptionSKA1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDQ96BD8
Purification>95%, Protein G purified
ImmunogenRecombinant Human Spindle and kinetochore-associated protein 1 protein (61-134AA)
StorageShipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesSpindle and kinetochore-associated protein 1, SKA1, C18orf24
ApplicationELISA, IHC; Recommended dilution: IHC: 1:200-1:500