BackgroundE3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
DescriptionSHPRH Polyclonal Antibody. Unconjugated. Raised in: Rabbit.
FormulationLiquid. 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
SpecificityHuman
IsotypeIgG
Uniprot IDQ149N8
Purification>95%, Protein G purified
ImmunogenRecombinant Human E3 ubiquitin-protein ligase SHPRH protein (1362-1512AA)
StorageShipped at 4°C. Upon receipt, store at -20°C (short-term) or -80°C (long-term). Avoid repeated freeze.
Alternative NamesE3 ubiquitin-protein ligase SHPRH, RING-type E3 ubiquitin transferase SHPRH, SNF2, histone-linker, PHD and RING finger domain-containing helicase, SHPRH, KIAA2023
ApplicationELISA, IHC, IF; Recommended dilution: IHC: 1:200-1:500, IF: 1:50-1:200